Key Takeaways What This Post Will Cover The Punchline: Buffered Doesn’t Always Mean Stable We use buffers to “lock in” the pH and control the ionization state of our analytes—but that control is fragile. You could have: And what shows up on your chromatogram? Common Buffer Pitfalls in Ionic Separations …
Main Takeaways What This Post Will Unpack The Punchline: The Method Didn’t Fail – The Chemistry Shifted You thought your buffer was stable. You measured pH 7.5. Everything was going smoothly—until you cranked the MeOH to 80% for a faster run and suddenly: But it wasn’t you—it was the solvent effect. …
Key Takeaways (For the Fast Readers) What This Post Will Unpack The Big Picture: How pH Impacts Retention Picture your analyte navigating a hallway. The walls? That’s your stationary phase (non-polar C18). The air around it? That’s your mobile phase. If the analyte is charged, it floats in the air …
In the sphere of analytical instrumentation, effective communication is key, and the language of instruments serves as a signal. For chemists, ensuring that the signal received from the ICP-OES instrument is clear, consistent, and concise becomes an utter priority. The sensitivity of the signal must be finely tuned to be …
In the US, roughly 16% of children experience some form of abuse, including physical, emotional, and sexual abuse. 18% of parents have a permissive parenting style which meets the clinical definition of bad parenting. Harsh parenting is associated with lower-control and higher aggressive attitude in children. Adolescents who have less self-control and stronger aggressive …
Have you ever had that exciting feeling that fresh graduates get when they think they totally nailed an interview that required more skills than they have as new grads? But then, months go by and you never hear back from the employer for the job? You are not alone, I …
The best way to measure your investing success is not by whether you’re beating the market but by whether you’ve put in place a financial plan and a behavioral discipline that are likely to get you where you want to go. – Benjamin Graham I am appalled by the fear …
You cannot strengthen one by weakening another; and you can’t increase the height of a dwarf by cutting off giant’s legs.” Benjamin franklin In January 1993, an unlabeled vial arrived at the white house. It was President Bill Clinton’s allergy medicine. The White House physician at the time Dr. Burton …
Why google is the best search engine out there? In my opinion, it is due to its capability of capturing the least amount of words provided by the user and comes up with the closest answer to your search. The same goes with analytical methods, one of the critical performance …
It is the ability of the method to obtain test results proportional to the concentration of analyte. What are the components of linearity? Figure 1 How to evaluate linearity? Figure 2 As per ICH guidelines Q2B, R1, the linearity should be evaluated initially by visual inspection of the response versus …
