🔑 Key Takeaways 🎯 The Punchline Think of MODR as your method’s “comfort zone” — a scientifically proven range where it works reliably, even if small changes happen. In traditional method development, you pick one set of conditions. You validate that.Then you hope everything else holds steady. In AQbD, you design a region, …
When working in regulated labs, you often hear phrases like: “Flow rate is a CQA…” “LOD is a CMP…” At first, those terms might sound correct. But in Analytical Quality by Design (AQbD), confusing Critical Quality Attributes (CQAs) with Critical Method Parameters (CMPs) is a common mistake. And it can be a costly …
Key Takeaways What This Post Will Cover The Punchline: If You Don’t Know the Target, You Can’t Hit It In analytical labs, too often, we jump straight into tweaking columns, mobile phases, and pH conditions. But if you don’t define what your method is supposed to achieve, you’re aiming at a …
Key Takeaways What This Post Will Cover 🎯 The Punchline: If You Don’t Know the Target, You Can’t Hit It In analytical labs, too often, we jump straight into tweaking columns, mobile phases, and pH conditions. But if you don’t define what your method is supposed to achieve, you’re aiming at a …
Key Takeaways What This Post Will Cover 🎯 The Punchline: Old-School Method Development Leaves Too Much to Chance In most labs—even good ones—analytical methods are developed like recipes passed down in a kitchen.Maybe it works. Maybe it doesn’t. But no one really knows why. Without structure, most methods: That’s where Analytical Quality by …
The punchline: In analytical chemistry, knowing how low you can go isn’t just academic—it’s essential. Your method’s limit of detection (LoD) and limit of quantification (LoQ) determine whether you can trust that tiny peak or that faint signal. Get these wrong, and you might miss a critical contaminant or report …
Key Takeaways What This Post Will Cover: 🎯 The Punchline: Peak Splitting is the Symptom—Not the Root Cause Whether it’s a buffer prep mistake, injector contamination, or unexpected pH drift, split peaks are a messenger, telling you something critical has shifted. You don’t fix split peaks—you fix the system causing them. 🧪 Part 1: Chemical Causes …
Key Takeaways What This Post Will Cover The Punchline: Buffered Doesn’t Always Mean Stable We use buffers to “lock in” the pH and control the ionization state of our analytes—but that control is fragile. You could have: And what shows up on your chromatogram? Common Buffer Pitfalls in Ionic Separations …
Main Takeaways What This Post Will Unpack The Punchline: The Method Didn’t Fail – The Chemistry Shifted You thought your buffer was stable. You measured pH 7.5. Everything was going smoothly—until you cranked the MeOH to 80% for a faster run and suddenly: But it wasn’t you—it was the solvent effect. …
Key Takeaways (For the Fast Readers) What This Post Will Unpack The Big Picture: How pH Impacts Retention Picture your analyte navigating a hallway. The walls? That’s your stationary phase (non-polar C18). The air around it? That’s your mobile phase. If the analyte is charged, it floats in the air …
