Why Buffer Choice Matters: Diagnosing and Preventing pH Drift in RP-HPLC

Key Takeaways What This Post Will Cover The Punchline: Buffered Doesn’t Always Mean Stable We use buffers to “lock in” the pH and control the ionization state of our analytes—but that control is fragile. You could have: And what shows up on your chromatogram? Common Buffer Pitfalls in Ionic Separations …

The pH Shift: Why Your Column Misbehaves in High Organic

Main Takeaways What This Post Will Unpack The Punchline: The Method Didn’t Fail – The Chemistry Shifted You thought your buffer was stable. You measured pH 7.5. Everything was going smoothly—until you cranked the MeOH to 80% for a faster run and suddenly: But it wasn’t you—it was the solvent effect. …

Why pH Matters in RP-HPLC: The Hidden Lever Behind Retention and Peak Shape

Key Takeaways (For the Fast Readers) What This Post Will Unpack The Big Picture: How pH Impacts Retention Picture your analyte navigating a hallway. The walls? That’s your stationary phase (non-polar C18). The air around it? That’s your mobile phase. If the analyte is charged, it floats in the air …

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