Why Buffer Choice Matters: Diagnosing and Preventing pH Drift in RP-HPLC

Key Takeaways What This Post Will Cover The Punchline: Buffered Doesn’t Always Mean Stable We use buffers to “lock in” the pH and control the ionization state of our analytes—but that control is fragile. You could have: And what shows up on your chromatogram? Common Buffer Pitfalls in Ionic Separations …

The pH Shift: Why Your Column Misbehaves in High Organic

Main Takeaways What This Post Will Unpack The Punchline: The Method Didn’t Fail – The Chemistry Shifted You thought your buffer was stable. You measured pH 7.5. Everything was going smoothly—until you cranked the MeOH to 80% for a faster run and suddenly: But it wasn’t you—it was the solvent effect. …

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